Applied Biosystems TaqMan MicroRNA Assays help researchers validate miRNA markers for forensic body fluid identification

Importance and challenges of identifying body fluids in forensic casework In forensic casework, correctly identifying body fluids such as blood, semen, or saliva is important for reconstructing a crime scene. Analysis of stains or biological fluids recovered during an investigation may provide potential sources of DNA that can be used to identify the donor of the fluid or stain [1]. However, body fluid samples can be difficult to work with, as often only limited quantities of sample are available at crime scenes. Moreover, samples may have been exposed to conditions that contribute to the degradation of RNA, and may yield only minute amounts of low-quality genetic material. Traditional serological or immunological methods of identifying body fluids can be costly in terms of time and labor, and require large amounts of sample. Moreover, these protein-based assays have varying degrees of sensitivity and specificity [2]. To be suitable for forensic casework, a body fluid identification assay must: